| First Author | McCoy DM | Year | 2006 |
| Journal | Am J Respir Cell Mol Biol | Volume | 35 |
| Issue | 3 | Pages | 394-402 |
| PubMed ID | 16645180 | Mgi Jnum | J:125248 |
| Mgi Id | MGI:3757923 | Doi | 10.1165/rcmb.2005-0401OC |
| Citation | McCoy DM, et al. (2006) Transcriptional regulation of lung cytidylyltransferase in developing transgenic mice. Am J Respir Cell Mol Biol 35(3):394-402 |
| abstractText | Lung development is associated with a surge in surfactant phosphatidylcholine (PC) production to prepare the newborn for extrauterine breathing. This process is associated with a marked increase in the activity of the rate-regulatory surfactant enzyme, CTP:phosphocholine cytidylyltransferase (CCTalpha). To investigate the molecular basis for developmental activation of CCTalpha, we analyzed expression of endogenous CCTalpha and a reporter gene, beta-galactosidase, in fetal, newborn, and adult promoter-reporter transgenic mice. Transgenics harboring approximately 2 kb of the CCTalpha promoter linked upstream of a beta-galactosidase reporter gene displayed relatively high expression in distal lung epithelia. Endogenous lung CCTalpha and beta-galactosidase activities, protein content, and transcript levels displayed maximal expression within the newborn period. CCTalpha and beta-galactosidase activities and enzyme levels increased with time in cultured fetal lung explants isolated from transgenics. Transfectional analysis using CCTalpha promoter-reporter constructs in developing rat type II cells revealed that a region encompassing -169/+71 contained the DNA elements required for perinatal activation. The studies demonstrate that developmental induction of surfactant phospholipid is due, at least in part, to transcriptional activation of the CCTalpha gene. |
