| First Author | Raich N | Year | 1990 |
| Journal | Science | Volume | 250 |
| Issue | 4984 | Pages | 1147-9 |
| PubMed ID | 2251502 | Mgi Jnum | J:127699 |
| Mgi Id | MGI:3764621 | Doi | 10.1126/science.2251502 |
| Citation | Raich N, et al. (1990) Autonomous developmental control of human embryonic globin gene switching in transgenic mice. Science 250(4984):1147-9 |
| abstractText | The mechanisms by which expression of the beta-like globin genes are developmentally regulated are under intense investigation. The temporal control of human embryonic (epsilon) globin expression was analyzed. A 3.7-kilobase (kb) fragment that contained the entire human epsilon-globin gene was linked to a 2.5-kb cassette of the locus control region (LCR), and the developmental time of expression of this construct was studied in transgenic mice. The human epsilon-globin transgene was expressed in yolk sac-derived primitive erythroid cells, but not in fetal liver or bone marrow-derived definitive erythroid cells. The absence of epsilon gene expression in definitive erythroid cells suggests that the developmental regulation of the epsilon-globin gene depends only on the presence of the LCR and the epsilon-globin gene itself (that is, an autonomous negative control mechanism). The autonomy of epsilon-globin gene developmental control distinguishes it from the competitive mechanism of regulation of gamma and beta-globin genes, and therefore, suggests that at least two distinct mechanisms function in human hemoglobin switching. |
