| First Author | Hayata T | Year | 2008 |
| Journal | Biochem Biophys Res Commun | Volume | 368 |
| Issue | 2 | Pages | 205-10 |
| PubMed ID | 18211825 | Mgi Jnum | J:136813 |
| Mgi Id | MGI:3797159 | Doi | 10.1016/j.bbrc.2008.01.040 |
| Citation | Hayata T, et al. (2008) Ciz, a transcription factor with a nucleocytoplasmic shuttling activity, interacts with C-propeptides of type I collagen. Biochem Biophys Res Commun 368(2):205-10 |
| abstractText | Ciz is a zinc finger transcription factor with nucleocytoplasmic shuttling activity. Ciz-deficient mice show high bone mass phenotype. As a first step to address how Ciz suppresses bone formation, we examined the binding partners of Ciz based on a yeast two-hybrid screening. While Ciz is an intracellular protein, 47% of the positive clones were genes encoding extracellular matrix proteins, including Col1a1, Col1a2, Fbln2, and Rpsa. In vitro coimmunoprecipitation experiments using in vitro translated proteins revealed direct binding of Ciz-DeltaZF (zinc finger) to C-propeptides of Col1a1 and Col1a2. In vivo association of the transfected Ciz and C-propeptide of Col1a1 was observed in COS-7 cells based on immunoprecipitation. In terms of intracellular localization, overexpressed C-propeptides of Col1 and Ciz were co-localized in nuclei. These results revealed that Ciz interacts with C-propeptides of type I collagen and this association takes place in nuclei. |
