| First Author | Warner N | Year | 2008 |
| Journal | FEBS J | Volume | 275 |
| Issue | 10 | Pages | 2561-73 |
| PubMed ID | 18422655 | Mgi Jnum | J:137549 |
| Mgi Id | MGI:3801222 | Doi | 10.1111/j.1742-4658.2008.06405.x |
| Citation | Warner N, et al. (2008) Analysis of EphA4 receptor tyrosine kinase substrate specificity using peptide-based arrays. FEBS J 275(10):2561-73 |
| abstractText | Eph receptor tyrosine kinases regulate many important biological processes. In the present study, we explored the substrate specificity of the EphA4 receptor tyrosine kinase using peptide arrays. We define a consensus substrate motif for EphA4 and go on to identify and test a number of potential EphA4 substrates and map their putative site(s) of phosphorylation. Cotransfection studies validate two of the predicted substrates: Nck2 and Dok1. Our findings identify several potential EphA4 substrates and demonstrate the general utility of using peptide arrays to rapidly identify and map protein kinase phosphorylation sites. |
