| First Author | Dalai SK | Year | 2008 |
| Journal | J Immunol | Volume | 181 |
| Issue | 5 | Pages | 3221-31 |
| PubMed ID | 18713993 | Mgi Jnum | J:138953 |
| Mgi Id | MGI:3806903 | Doi | 10.4049/jimmunol.181.5.3221 |
| Citation | Dalai SK, et al. (2008) Anergy in memory CD4+ T cells is induced by B cells. J Immunol 181(5):3221-31 |
| abstractText | Induction of tolerance in memory T cells has profound implications in the treatment of autoimmune diseases and transplant rejection. Previously, we reported that the presentation of low densities of agonist peptide/MHC class II complexes induced anergy in memory CD4(+) T cells. In the present study, we address the specific interaction of different types of APCs with memory CD4(+) T cells. A novel ex vivo anergy assay first suggested that B cells induce anergy in memory T cells, and an in vivo cell transfer assay further confirmed those observations. We demonstrated that B cells pulsed with defined doses of Ag anergize memory CD4 cells in vivo. We established that CD11c(+) dendritic cells do not contribute to anergy induction to CD4 memory T cells, because diphtheria toxin receptor-transgenic mice that were conditionally depleted of dendritic cells optimally induced anergy in memory CD4(+) T cells. Moreover, B cell-deficient muMT mice did not induce anergy in memory T cells. We showed that B2 follicular B cells are the specific subpopulation of B cells that render memory T cells anergic. Furthermore, we present data showing that anergy in this system is mediated by CTLA-4 up-regulation on T cells. This is the first study to demonstrate formally that B cells are the APCs that induce anergy in memory CD4(+) T cells. |
