| First Author | Carpentier I | Year | 2008 |
| Journal | Biochem Biophys Res Commun | Volume | 374 |
| Issue | 4 | Pages | 752-7 |
| PubMed ID | 18671942 | Mgi Jnum | J:140049 |
| Mgi Id | MGI:3811679 | Doi | 10.1016/j.bbrc.2008.07.103 |
| Citation | Carpentier I, et al. (2008) Smurf2 is a TRAF2 binding protein that triggers TNF-R2 ubiquitination and TNF-R2-induced JNK activation. Biochem Biophys Res Commun 374(4):752-7 |
| abstractText | TRAF2 plays a central role in TNF-induced signalling to NF-kappaB and JNK/p38 MAPK. To better understand the molecular mechanisms that mediate this dual function of TRAF2, we performed a yeast two-hybrid screening for TRAF2 interacting proteins using the Sos recruitment system. This resulted in the identification of the E3 ubiquitin ligase Smurf2 as a TRAF2 binding protein. TRAF2 overexpression was shown to trigger Smurf2 ubiquitination and the formation of a TNF-R2/Smurf2 complex. Smurf2 on its turn promoted TNF-R2 ubiquitination and the relocalization of TNF-R2 as well as TRAF2 to a detergent-insoluble cell fraction. This was associated with enhanced TNF-R2-induced JNK activation, whereas TNF-R2-induced NF-kappaB activation remained unaffected. These results suggest an important role for Smurf2 binding to TRAF2 in determining specific signalling outputs of TNF-R2. |
