| First Author | Wang HV | Year | 2008 |
| Journal | Dev Dyn | Volume | 237 |
| Issue | 11 | Pages | 3342-51 |
| PubMed ID | 18924229 | Mgi Jnum | J:140659 |
| Mgi Id | MGI:3814274 | Doi | 10.1002/dvdy.21755 |
| Citation | Wang HV, et al. (2008) Comparative expression analysis of the murine palladin isoforms. Dev Dyn 237(11):3342-51 |
| abstractText | Palladin fulfils a crucial function as a molecular scaffold in organizing and stabilizing the actin cytoskeleton. At least four major palladin isoforms exist due to different promoter usage and alternative splicing: a 200-kDa isoform, a 140-kDa isoform, and two isoforms with a size of 90-92 kDa. Here, we describe their expression during mouse development and in adult tissues. The 200-kDa isoform is predominantly expressed in developing heart and skeletal muscle. The 140-kDa isoform is expressed in various mesenchymal tissues, and also represents the major isoform of the brain. The 90-92-kDa isoforms are almost ubiquitously expressed with the highest levels in smooth muscle-rich tissues. Immunohistochemical and immunofluorecence staining with an anti-200-kDa isoform-specific antiserum localizes the large isoform to the Z-discs of cardiac and skeletal muscle cells. Interestingly, the expression of this isoform is initiated and increasing during in vitro differentiation and fusion of C2C12 myoblasts, suggesting that the 200-kDa palladin isoform may play a scaffolding role during sarcomeric organization. Developmental Dynamics 237:3342-3351, 2008. (c) 2008 Wiley-Liss, Inc. |
