| First Author | Salomon SN | Year | 2008 |
| Journal | Neurosci Lett | Volume | 447 |
| Issue | 1 | Pages | 62-7 |
| PubMed ID | 18832009 | Mgi Jnum | J:143458 |
| Mgi Id | MGI:3826947 | Doi | 10.1016/j.neulet.2008.09.051 |
| Citation | Salomon SN, et al. (2008) Localization of the Diaphanous-related formin Daam1 to neuronal dendrites. Neurosci Lett 447(1):62-7 |
| abstractText | The Rho family of small GTPase proteins are involved in the formation and maintenance of neuronal dendrites. In this study, we show that Daam1, a member of the Diaphanous-related formin protein family and a downstream effector for RhoA, is localized to the dendrites of hippocampal neurons. Immunoblot analysis showed that Daam1 is enriched in the mouse hippocampus and co-fractionates in brain lysates with dendritic and synaptic proteins. Immunohistochemical analysis revealed that Daam1 protein distributes in a punctate pattern throughout the cell body and dendritic shafts of dissociated hippocampal neurons and organotypic hippocampal cultures. Although Daam1 is mostly expressed in the shaft of dendrites, co-stainings with SV2 or PSD95 revealed that Daam1 is also present at some synapses. In addition, viral directed expression of a fluorescently tagged Daam1 fusion protein in hippocampal slices resulted in targeted delivery to the dendrites of pyramidal neurons, leading to a reduction in the density of spines. |
