| First Author | Osman MS | Year | 2007 |
| Journal | J Immunol | Volume | 178 |
| Issue | 3 | Pages | 1261-7 |
| PubMed ID | 17237371 | Mgi Jnum | J:143665 |
| Mgi Id | MGI:3828385 | Doi | 10.4049/jimmunol.178.3.1261 |
| Citation | Osman MS, et al. (2007) Activating Ly-49 receptors regulate LFA-1-mediated adhesion by NK cells. J Immunol 178(3):1261-7 |
| abstractText | NK cells are important for innate resistance to tumors and viruses. Engagement of activating Ly-49 receptors expressed by NK cells leads to rapid NK cell activation resulting in target cell lysis and cytokine production. The ITAM-containing DAP12 adapter protein stably associates with activating Ly-49 receptors, and couples receptor recognition with generation of NK responses. Activating Ly-49s are potent stimulators of murine NK cell functions, yet how they mediate such activities is not well understood. We demonstrate that these receptors trigger LFA-1-dependent tight conjugation between NK cells and target cells. Furthermore, we show that activating Ly-49 receptor engagement leads to rapid DAP12-dependent up-regulation of NK cell LFA-1 adhesiveness to ICAM-1 that is also dependent on tyrosine kinases of the Syk and Src families. These results indicate for the first time that activating Ly-49s control adhesive properties of LFA-1, and by DAP12-dependent inside-out signaling. Ly-49-driven mobilization of LFA-1 adhesive function may represent a fundamental proximal event during NK cell interactions with target cells involving activating Ly-49 receptors, leading to target cell death. |
