| First Author | Toma-Hirano M | Year | 2009 |
| Journal | Eur J Immunol | Volume | 39 |
| Issue | 5 | Pages | 1344-52 |
| PubMed ID | 19350550 | Mgi Jnum | J:148098 |
| Mgi Id | MGI:3843540 | Doi | 10.1002/eji.200838363 |
| Citation | Toma-Hirano M, et al. (2009) Ly49Q ligand expressed by activated B cells induces plasmacytoid DC maturation. Eur J Immunol 39(5):1344-52 |
| abstractText | Ly49Q, a type II C-type lectin expressed on mouse plasmacytoid DC (pDC), contains a single carbohydrate recognition domain in its extracellular region and an ITIM in its cytoplasmic domain. We have identified the MHC class I molecule H-2K(b) as a Ly49Q ligand, confirming prior reports. Although H-2K(b) is expressed on essentially all hematopoietic cells, we found that only CpG-stimulated B cells were able to activate Ly49Q. This discovery correlated with our finding that although H-2K(b) forms clusters on CpG-activated B cells, it is diffusely expressed on resting B cells. Furthermore, CpG-stimulated, but not resting, B cells up-regulated co-stimulatory molecules on pDC. This finding was confirmed by the fact that binding by anti-Ly49Q mAb to Ly49Q led to pDC maturation in vitro. Our results suggest that clustered H-2K(b) on activated B cells act as ligands for Ly49Q and induce pDC maturation in vitro. |
