| First Author | Moschetti T | Year | 2009 |
| Journal | Biochem Biophys Res Commun | Volume | 390 |
| Issue | 1 | Pages | 121-4 |
| PubMed ID | 19782043 | Mgi Jnum | J:155133 |
| Mgi Id | MGI:4412335 | Doi | 10.1016/j.bbrc.2009.09.078 |
| Citation | Moschetti T, et al. (2009) Failure of apoptosis-inducing factor to act as neuroglobin reductase. Biochem Biophys Res Commun 390(1):121-4 |
| abstractText | Neuroglobin (Ngb) is a hexacoordinate globin expressed in the nervous system of vertebrates, where it protects neurons against hypoxia. Ferrous Ngb has been proposed to favor cell survival by scavenging NO and/or reducing cytochrome c released into the cytosol during hypoxic stress. Both catalytic functions require an as yet unidentified Ngb-reductase activity. Such an activity was detected both in tissue homogenates of human brain and liver and in Escherichia coli extracts. Since NADH:flavorubredoxin oxidoreductase from E. coli, that was shown to reduce ferric Ngb, shares sequence similarity with the human apoptosis-inducing factor (AIF), AIF has been proposed by us as a candidate Ngb reductase. In this study, we tested this hypothesis and show that the Ngb-reductase activity of recombinant human AIF is negligible and hence incompatible with such a physiological function. |
