| First Author | Yang X | Year | 2010 |
| Journal | FEBS Lett | Volume | 584 |
| Issue | 5 | Pages | 903-10 |
| PubMed ID | 20083112 | Mgi Jnum | J:157594 |
| Mgi Id | MGI:4431160 | Doi | 10.1016/j.febslet.2009.12.058 |
| Citation | Yang X, et al. (2010) Identification of a novel splicing isoform of murine CGI-58. FEBS Lett 584(5):903-10 |
| abstractText | The comparative gene identification-58 (CGI-58) gene, mutations of which are linked to Chanarin-Dorfman syndrome, encodes a protein of the alpha/beta hydrolase domain subfamily. We report here a new alternative splicing isoform of the murine CGI-58 gene, termed mCGI-58S. Sequence comparison indicates the lack of second and third exons in this cDNA variant. While the full-length protein displayed perilipin-dependent localization to lipid droplets, mCGI-58S showed a predominant cytoplasmic staining when expressed in cells. mCGI-58S was incapable of activating adipose triglyceride lipase but retained the capacity to acylate lysophosphatidic acid. Overexpression of mCGI-58S failed to promote lipid droplet turnover and loss of intracellular triacylglycerols. These results suggest that this splicing event may be involved in the regulation of lipid homeostasis. |
