| First Author | Nalbant P | Year | 2004 |
| Journal | Science | Volume | 305 |
| Issue | 5690 | Pages | 1615-9 |
| PubMed ID | 15361624 | Mgi Jnum | J:159066 |
| Mgi Id | MGI:4441125 | Doi | 10.1126/science.1100367 |
| Citation | Nalbant P, et al. (2004) Activation of endogenous Cdc42 visualized in living cells. Science 305(5690):1615-9 |
| abstractText | Signaling proteins are tightly regulated spatially and temporally to perform multiple functions. For Cdc42 and other guanosine triphosphatases, the subcellular location of activation is a critical determinant of cell behavior. However, current approaches are limited in their ability to examine the dynamics of Cdc42 activity in living cells. We report the development of a biosensor capable of visualizing the changing activation of endogenous, unlabeled Cdc42 in living cells. With the use of a dye that reports protein interactions, the biosensor revealed localized activation in the trans-Golgi apparatus, microtubule-dependent Cdc42 activation at the cell periphery, and activation kinetics precisely coordinated with cell extension and retraction. |
