| First Author | Kimura T | Year | 2010 |
| Journal | Biochem Biophys Res Commun | Volume | 395 |
| Issue | 3 | Pages | 318-23 |
| PubMed ID | 20362548 | Mgi Jnum | J:160347 |
| Mgi Id | MGI:4454256 | Doi | 10.1016/j.bbrc.2010.03.173 |
| Citation | Kimura T, et al. (2010) Glucose-induced translocation of coronin 3 regulates the retrograde transport of the secretory membrane in the pancreatic beta-cells. Biochem Biophys Res Commun 395(3):318-23 |
| abstractText | GTP-Rab27a is known to regulate insulin exocytosis. We have recently reported that coronin 3, which paradoxically binds GDP-Rab27a, participates in endocytosis of the insulin secretory membrane. Here, we demonstrate that glucose stimulation caused redistribution of coronin 3 in the vicinity of the plasma membrane, which was mimicked by overexpression of the GDP-Rab27a mutant or the Rab27a GAP. Glucose-induced translocation of coronin 3 was inhibited by Rab27a knock-down. The internalized phogrin, an insulin granule associated protein, located near the plasma membrane by the dominant-negative coronin 3, but the protein at the outer surface of the plasma membrane was decreased. These results indicate that glucose recruits coronin 3 near the plasma membrane, and that it regulates the retrograde transport of the secretory membrane in pancreatic beta-cells. |
