|  Help  |  About  |  Contact Us

Publication : Stimulatory actions of lysophosphatidic acid on mouse ATDC5 chondroprogenitor cells.

First Author  Itoh R Year  2010
Journal  J Bone Miner Metab Volume  28
Issue  6 Pages  659-71
PubMed ID  20458606 Mgi Jnum  J:166492
Mgi Id  MGI:4845836 Doi  10.1007/s00774-010-0184-1
Citation  Itoh R, et al. (2010) Stimulatory actions of lysophosphatidic acid on mouse ATDC5 chondroprogenitor cells. J Bone Miner Metab 28(6):659-71
abstractText  Lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are bioactive lysophospholipids that affect various cellular processes through G protein-coupled receptors. In our current study, we found by in situ hybridization that E11.5 mouse embryos strongly expressed the LPA receptor subtype LPA(1) in cartilaginous bone primordia and the surrounding mesenchymal cells. However, despite their wide-ranging actions, the roles of lysophospholipids in chondrogenesis remain poorly understood. The mouse clonal cell line ATDC5 undergoes a sequential differentiation of chondroprogenitor cells in vitro. Undifferentiated and differentiated ATDC5 cells express LPA(1) and other lysophospholipid receptors including S1P receptor S1P(1) and S1P(2). Taking advantage of this cell model, we studied the effects of LPA on the activities of chondroprogenitor cells. LPA markedly stimulates both DNA synthesis and the migration of ATDC5 chondroprogenitor cells in culture, whereas S1P suppresses the migration of these cells. Treatment with Ki16425, an LPA(1)- and LPA(3)-specific receptor antagonist, suppressed the fetal bovine serum-stimulated migration of ATDC5 cells by almost 80%. These results indicate that LPA plays an important role in the activation of chondroprogenitor cells.
Quick Links:
 
Quick Links:
 

Expression

Publication --> Expression annotations

 

Other

12 Bio Entities

45 Expression