| First Author | Yan XL | Year | 2011 |
| Journal | Dev Dyn | Volume | 240 |
| Issue | 1 | Pages | 65-74 |
| PubMed ID | 21089075 | Mgi Jnum | J:167615 |
| Mgi Id | MGI:4868643 | Doi | 10.1002/dvdy.22490 |
| Citation | Yan XL, et al. (2011) Migration of dorsal aorta mesenchymal stem cells induced by mouse embryonic circulation. Dev Dyn 240(1):65-74 |
| abstractText | Mesenchymal stem cells (MSCs) represent powerful tools for regenerative medicine for their differentiation and migration capacity. However, ontogeny and migration of MSCs in mammalian mid-gestation conceptus is poorly understood. We identified canonical MSCs in the mouse embryonic day (E) 11.5 dorsal aorta (DA). They possessed homogenous immunophenotype (CD45(-)CD31(-)Flk-1(-)CD44(+)CD29(+)), expressed perivascular markers (alpha-SMA(+)NG2(+)PDGFRbeta(+)PDGFRalpha(+)), and had tri-lineage differentiation potential (osteoblasts, adipocytes, and chondrocytes). Of interest, MSCs were also detected in E12.5-E13.5 embryonic circulation, 24 hr later than in DA, suggesting migration like hematopoietic stem cells. Functionally, E12.5 embryonic blood could trigger efficient migration of DA-MSCs through platelet-derived growth factor (PDGF) receptor-, transforming growth factor-beta receptor-, but not basic fibroblast growth factor receptor-mediated signaling. Moreover, downstream JNK and AKT signaling pathway played important roles in embryonic blood- or PDGF-mediated migration of DA-derived MSCs. Taken together, these results revealed that clonal MSCs developed in the mouse DA. More importantly, the embryonic circulation, in addition to its conventional transporting roles, could modulate migration of MSC during early embryogenesis. |
