| First Author | Zhang L | Year | 2011 |
| Journal | Genes Dev | Volume | 25 |
| Issue | 2 | Pages | 119-24 |
| PubMed ID | 21196494 | Mgi Jnum | J:167985 |
| Mgi Id | MGI:4881573 | Doi | 10.1101/gad.1998711 |
| Citation | Zhang L, et al. (2011) miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1. Genes Dev 25(2):119-24 |
| abstractText | Using RNA-seq technology, we found that the majority of microRNAs (miRNAs) present in CFU-E erythroid progenitors are down-regulated during terminal erythroid differentiation. Of the developmentally down-regulated miRNAs, ectopic overexpression of miR-191 blocks erythroid enucleation but has minor effects on proliferation and differentiation. We identified two erythroid-enriched and developmentally up-regulated genes, Riok3 and Mxi1, as direct targets of miR-191. Knockdown of either Riok3 or Mxi1 blocks enucleation, and either physiological overexpression of miR-191 or knockdown of Riok3 or Mxi1 blocks chromatin condensation. Thus, down-regulation of miR-191 is essential for erythroid chromatin condensation and enucleation by allowing up-regulation of Riok3 and Mxi1. |
