| First Author | Celada A | Year | 1996 |
| Journal | Biochem J | Volume | 313 ( Pt 3) |
| Pages | 737-44 | PubMed ID | 8611149 |
| Mgi Jnum | J:169819 | Mgi Id | MGI:4942272 |
| Doi | 10.1042/bj3130737 | Citation | Celada A, et al. (1996) Identification of a transcription factor that binds to the S box of the I-A beta gene of the major histocompatibility complex. Biochem J 313(Pt 3):737-44 |
| abstractText | Class II genes of the MHC show a striking homology upstream of the transcription start site that is composed of three conserved sequences (S, X and Y boxes, each separated by 15-20 bp). The presence of the S-box sequence in the mouse MHC class II gene I-A Beta was examined for its influence on the expression of this gene. Deletion or mutation of the S box decreased the induction of chloramphenicol acetyltransferase (CAT) activity in B lymphocytes by 32%. In macrophages, deletion or mutation of the S box abolished interferon-gamma (IFN-gamma) inducibility of CAT activity. Using a gel-retardation assay, we have identified a nuclear factor whose binding site overlaps the 7-mer conserved sequence of the S box. This factor is present in lymphocytes, macrophages, mastocytes and fibroblasts. Surprisingly, binding of this nuclear factor to DNA was induced by IFN-gamma in bone-marrow-derived macrophages, but not in macrophage-like cell lines. The binding site for this factor was defined by DNase I footprinting and partially purified by using an affinity column containing double-stranded oligonucleotides containing a sequence of the S box. A prominent protein of 43 kDa was found that bound specifically to the S-box sequence. |
