| First Author | Hernandez MC | Year | 2010 |
| Journal | Dev Neurosci | Volume | 32 |
| Issue | 4 | Pages | 278-87 |
| PubMed ID | 20805681 | Mgi Jnum | J:170220 |
| Mgi Id | MGI:4944155 | Doi | 10.1159/000314723 |
| Citation | Hernandez MC, et al. (2010) Quantitative mRNA analysis of serotonin 5-HT and adrenergic beta receptors in the mouse embryonic telencephalon. Dev Neurosci 32(4):278-87 |
| abstractText | The adrenergic beta receptor (beta-AR) gene is embedded (nested) within the serotonin 5-HT receptor (5-HT-R) gene and these two receptors can interact at the transcriptional and post-transcriptional levels. The mouse 5-HT-R gene contains a number of exons and codes at least four mRNA splice variants (5-HT(4(a))-R, 5-HT(4(b))-R, 5-HT(4(e))-R, 5-HT(4(f))-R), whereas the beta-AR gene is intronless. Since 5-HT-Rs and beta-ARs can form homodimers and heterodimers and they increase intracellular cAMP levels, these receptors may be important for integrating serotonergic and noradrenergic signals at the single-neuron level. Both 5-HT-R and beta-AR have been implicated in autism spectrum disorders, depression, and Alzheimer's disease. In the fetal brain, these receptors may mediate the effects of stress on neurodevelopmental processes. We used quantitative reverse-transcription PCR (qRT-PCR) to investigate the developmental expression of 5-HT-R and beta-AR in the mouse telencephalon at embryonic days (E) 13-18. At E13-E14, the mRNA levels of all 5-HT-R splice variants were very low, but by E17-E18 they increased 7-fold (5- HT(4(a))-R), 5-fold (5-HT(4(b))-R), 9-fold (5-HT(4(e))-R), and 11-fold (5-HT(4(f))-R). The expression of 5-HT(4(a))-R and 5-HT(4(b))-R was rapidly upregulated between E14 and E15, at the time when the thalamocortical projections arrive in the telencephalon. This pattern was not observed in the expression of 5-HT(4(e))-R and 5-HT(4(f))-R, the mRNA levels of which showed a steady, gradual increase from E13 to E18. The beta-AR mRNA levels were relatively high throughout the studied period of development and increased only by 70% from E13-E14 to E17-E18. These findings suggest that 5-HT-R splice variants and beta-ARs are differentially regulated in the embryonic telencephalon and that their relative amounts may carry developmentally important information. |
