| First Author | Lee YB | Year | 2009 |
| Journal | Nucleic Acids Res | Volume | 37 |
| Issue | 1 | Pages | 123-8 |
| PubMed ID | 19029138 | Mgi Jnum | J:173045 |
| Mgi Id | MGI:5009506 | Doi | 10.1093/nar/gkn920 |
| Citation | Lee YB, et al. (2009) Twist-1 regulates the miR-199a/214 cluster during development. Nucleic Acids Res 37(1):123-8 |
| abstractText | MicroRNAs are known to regulate developmental processes but their mechanism of regulation remains largely uncharacterized. We show the transcription factor Twist-1 drives the expression of a 7.9-kb noncoding RNA transcript (from the Dynamin-3 gene intron) that encodes a miR-199a and miR-214 cluster. We also show that knocking down Twist-1 with shRNAs decreased miR-199a/214 levels and that Twist-1 bound an E-Box promoter motif to developmentally regulate the expression of these miRNAs. The expression of HIF-1 (known to mediate Twist-1 transcription), miR-199a and miR-214 was maximal at E12.5 and the miRNAs were expressed specifically in mouse cerebellum, midbrain, nasal process and fore- and hindlimb buds. This study shows the expression of the miR199a/214 cluster is controlled by Twist-1 via an E-Box promoter element and supports a role for these miRNAs as novel intermediates in the pathways controlling the development of specific neural cell populations. |
