| First Author | Feng SY | Year | 2010 |
| Journal | Nucleic Acids Res | Volume | 38 |
| Issue | 20 | Pages | e189 |
| PubMed ID | 20798175 | Mgi Jnum | J:173818 |
| Mgi Id | MGI:5050392 | Doi | 10.1093/nar/gkq757 |
| Citation | Feng SY, et al. (2010) A yeast one-hybrid system to screen for methylated DNA-binding proteins. Nucleic Acids Res 38(20):e189 |
| abstractText | We had previously exploited a method for targeted DNA methylation in budding yeast to succeed in one-hybrid detection of methylation-dependent DNA-protein interactions. Based on this finding, we developed a yeast one-hybrid system to screen cDNA libraries for clones encoding methylated DNA-binding proteins. Concurrent use of two independent bait sequences in the same cell, or dual-bait system, effectively reduced false positive clones, which were derived from methylation-insensitive sequence-specific DNA-binding proteins. We applied the dual-bait system to screen cDNA libraries and demonstrated efficient isolation of clones for methylated DNA-binding proteins. This system would serve as a unique research tool for epigenetics. |
