| First Author | Mosquera J | Year | 2011 |
| Journal | Biochem Biophys Res Commun | Volume | 409 |
| Issue | 3 | Pages | 539-43 |
| PubMed ID | 21600873 | Mgi Jnum | J:174083 |
| Mgi Id | MGI:5051869 | Doi | 10.1016/j.bbrc.2011.05.040 |
| Citation | Mosquera J, et al. (2011) Identification of Ski as a target for Aurora A kinase. Biochem Biophys Res Commun 409(3):539-43 |
| abstractText | Ski is a negative regulator of the transforming growth factor-beta and other signalling pathways. The absence of SKI in mouse fibroblasts leads to chromosome segregation defects and genomic instability, suggesting a role for Ski during mitosis. At this stage, Ski is phosphorylated but to date little is known about the kinases involved in this process. Here, we show that Aurora A kinase is able to phosphorylate Ski in vitro. In vivo, Aurora A and Ski co-localized at the centrosomes and co-immunoprecipitated. Conversely, a C-terminal truncation mutant of Ski (SkiDelta491-728) lacking a coiled-coil domain, displayed decreased centrosomal localization. This mutant no longer co-immunoprecipitated with Aurora-A in vivo, but was still phosphorylated in vitro, indicating that the Ski-Aurora A interaction takes place at the centrosomes. These data identify Ski as a novel target of Aurora A and contribute to an understanding of the role of these proteins in the mitotic process. |
