| First Author | Zhu L | Year | 2011 |
| Journal | Int Immunol | Volume | 23 |
| Issue | 9 | Pages | 545-51 |
| PubMed ID | 21727177 | Mgi Jnum | J:175897 |
| Mgi Id | MGI:5287910 | Doi | 10.1093/intimm/dxr049 |
| Citation | Zhu L, et al. (2011) Excessive amounts of mu heavy chain block B-cell development. Int Immunol 23(9):545-51 |
| abstractText | Antigen-independent B-cell development occurs in several stages that depend on the expression of Ig heavy and light chain. We identified a line of mice that lacked mature B cells in the spleen. This mouse line carried approximately 11 copies of a transgene of the murine heavy chain constant region locus, and B-lineage cells expressed excessive amounts of the intracellular mu heavy chain. B-cell development failed in the bone marrow at the pro/pre B-cell transition, and examination of other lines with various copy numbers of the same transgene suggested that deficiencies in B-cell development increased with increased transgene copy number. Expression of a transgenic (Tg) light chain along with the Tg mu heavy chain led to minimal rescue of B-cell development in the bone marrow and B cells in the spleen. There are several potential mechanisms for the death of pro/pre B cells as a consequence of excess heavy chain expression. |
