| First Author | Baum O | Year | 2011 |
| Journal | FEBS Lett | Volume | 585 |
| Issue | 20 | Pages | 3219-23 |
| PubMed ID | 21945318 | Mgi Jnum | J:177345 |
| Mgi Id | MGI:5294841 | Doi | 10.1016/j.febslet.2011.09.016 |
| Citation | Baum O, et al. (2011) The beta-isoform of neuronal nitric oxide synthase (nNOS) lacking the PDZ domain is localized at the sarcolemma. FEBS Lett 585(20):3219-23 |
| abstractText | In skeletal muscles, the expression of neuronal NO synthase (nNOS) isoforms is uncharacterized at the protein level. We therefore conducted epitope mapping with anti-peptide-antibodies. Antibodies specific for the nNOS N-terminus recognized the 160-kDa alpha-isoform. In contrast, antibodies against the middle portion or the C-terminus of nNOS bound additionally to the truncated 140-kDa beta-isoform which lacks the PDZ-domain present in the alpha-isoform. All nNOS immunohistochemical reactivity was confined to the sarcolemma. Consistently, immunoblotting disclosed both nNOS-isoforms to be co-enriched in the membrane-associated fractions. The beta-isoform was co-immunoprecipitated with alpha-isoform antibodies in muscle extracts indicating an association of both nNOS-isoforms to direct the beta-variant to the sarcolemma. |
