| First Author | Kanamoto T | Year | 2011 |
| Journal | Curr Eye Res | Volume | 36 |
| Issue | 1 | Pages | 66-70 |
| PubMed ID | 21174600 | Mgi Jnum | J:179795 |
| Mgi Id | MGI:5303056 | Doi | 10.3109/02713683.2010.514658 |
| Citation | Kanamoto T, et al. (2011) Cullin5 reduces retinal cell death induced by glutamate toxicity. Curr Eye Res 36(1):66-70 |
| abstractText | PURPOSE: To investigate the expression in the mouse retina and the function in a retinal cell of cullin5 that is known as a protein that is associated with ubiquitilation. METHODS: The retinal sites of expressing cullin5 were determined by immunohistochemistry with a specific antibody for cullin5. Retinal cells were transfected with HA-tagged cullin5 using an adenovirus system, and retinal cells were exposed to L-glutamate with or without an over-expression of cullin5. RESULTS: Cullin5 was expressed in the retina of C57BL/6N mice and the retinal ganglion cell layer (RGL) of retina was strongly immunostained. Further, cullin5 was localized in the retinal ganglion cell (RGC). An over-expression of cullin5 reduced the percentage of retinal cell deaths induced by L-glutamate. CONCLUSIONS: The expression of cullin5 on retinal cells and reduction in the percentage of dead retinal cells induced by L-glutamate suggest that cullin5 has neuroprotective properties in retinal cells. |
