| First Author | Yao LH | Year | 2012 |
| Journal | J Neurosci | Volume | 32 |
| Issue | 11 | Pages | 3778-85 |
| PubMed ID | 22423098 | Mgi Jnum | J:183081 |
| Mgi Id | MGI:5317465 | Doi | 10.1523/JNEUROSCI.3540-11.2012 |
| Citation | Yao LH, et al. (2012) Synaptotagmin 1 is necessary for the Ca2+ dependence of clathrin-mediated endocytosis. J Neurosci 32(11):3778-85 |
| abstractText | The role of Ca(2) in synaptic vesicle endocytosis remains uncertain due to the diversity in various preparations where several forms of endocytosis may contribute variably in different conditions. Although recent studies have demonstrated that Ca(2) is important for clathrin-mediated endocytosis (CME), the mechanistic role of Ca(2) in CME remains to be elucidated. By monitoring CME of single vesicles in mouse chromaffin cells with cell-attached capacitance measurements that offer millisecond time resolution, we demonstrate that the dynamics of vesicle fission during CME is Ca(2) dependent but becomes Ca(2) independent in synaptotagmin 1 (Syt1) knock-out cells. Our results thus suggest that Syt1 is necessary for the Ca(2) dependence of CME. |
