| First Author | Bashtrykov P | Year | 2012 |
| Journal | FEBS Lett | Volume | 586 |
| Issue | 13 | Pages | 1821-3 |
| PubMed ID | 22641038 | Mgi Jnum | J:185525 |
| Mgi Id | MGI:5429111 | Doi | 10.1016/j.febslet.2012.05.026 |
| Citation | Bashtrykov P, et al. (2012) Mechanistic details of the DNA recognition by the Dnmt1 DNA methyltransferase. FEBS Lett 586(13):1821-3 |
| abstractText | A recently solved Dnmt1-DNA crystal structure revealed several enzyme-DNA contacts and large structural rearrangements of the DNA at the target site, including the flipping of the non-target strand base of the base pair flanking the CpG site and formation of a non-canonical base pair between the non-target strand Gua and the flanking base pair. Here, we show that the contacts of the enzyme to the target base and the Gua:5mC base pair that are observed in the structure are very important for catalytic activity. The contacts to the non-target strand Gua are not important since its exchange by Ade stimulated activity. Except target base flipping, we could not find evidence that the DNA rearrangements have a functional role. |
