| First Author | Cheng Y | Year | 2012 |
| Journal | Mol Reprod Dev | Volume | 79 |
| Issue | 8 | Pages | 573-85 |
| PubMed ID | 22730016 | Mgi Jnum | J:186549 |
| Mgi Id | MGI:5432626 | Doi | 10.1002/mrd.22063 |
| Citation | Cheng Y, et al. (2012) Hybrid expression cassettes consisting of a milk protein promoter and a cytomegalovirus enhancer significantly increase mammary-specific expression of human lactoferrin in transgenic mice. Mol Reprod Dev 79(8):573-85 |
| abstractText | It is very important to develop an effective, specific, and robust expression cassette that ensures a high level of expression in the mammary glands. In this study, we designed and constructed a series of mammary gland-specific vectors containing a complex hybrid promoter/enhancer by utilizing promoter sequences from milk proteins (i.e., goat beta-casein, bovine alphas1-casein, or goat beta-lactoglobulin) and cytomegalovirus enhancer sequences; vectors containing a single milk protein promoter served as controls. Chicken beta-globin insulator sequences were also included in some of these vectors. The expression of constructs was analyzed through the generation of transgenic mice. Enzyme-linked immunosorbent assay (ELISA) analysis revealed that the hybrid promoter/enhancer could drive the expression of recombinant human lactoferrin (rhLF) cDNA at high levels (1.17-8.10 mg/ml) in the milk of transgenic mice, whereas control promoters achieved a very low rhLF expression (7-40 ng/ml). Moreover, the expression of rhLF was not detected in the serum or saliva of any transgenic animal. This result shows that all constructs, driven by the hybrid promoter/enhancer, had high mammary gland-specific expression pattern. Together, our results suggest that the use of a hybrid promoter/enhancer is a valuable alternative approach for increasing mammary-specific expression of recombinant hLF in a transgenic mouse model.Mol. Reprod. Dev. 79: 573-585, 2012. (c) 2012 Wiley Periodicals, Inc. |
