| First Author | Tsukasaki M | Year | 2012 |
| Journal | Biochem Biophys Res Commun | Volume | 425 |
| Issue | 2 | Pages | 390-2 |
| PubMed ID | 22842459 | Mgi Jnum | J:187161 |
| Mgi Id | MGI:5435622 | Doi | 10.1016/j.bbrc.2012.07.106 |
| Citation | Tsukasaki M, et al. (2012) Nephronectin expression is regulated by SMAD signaling in osteoblast-like MC3T3-E1 cells. Biochem Biophys Res Commun 425(2):390-2 |
| abstractText | Nephronectin (Npnt) is an extracellular matrix protein known to be a ligand for the integrin alpha8beta1. We previously demonstrated that Npnt expression was suppressed by TGF-beta through ERK1/2 and JNK in osteoblasts. In this study, we found that inhibition of a TGF-beta type I receptor (TGF-beta R1, Alk5) by a specific inhibitor {2-[3-(6-Methylpyridin-2-yl)-1H-pyrazol-4-yl]-1,5-naphthyridine} strongly induced Npnt expression in osteoblast-like MC3T3-E1 cells. The Alk5 inhibitor-induced increase of Npnt expression occurred in both time- and dose-dependent manners, while that expression was also induced by introduction of an siRNA for Smad2, a central intracellular mediator of TGF-beta signaling. These results suggest that the expression of Npnt is regulated by the Alk5-SMAD signaling pathway in osteoblasts. |
