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Publication : Immunocytochemical study of YB-1 nuclear distribution in different cell types.

First Author  Bogolyubova IO Year  2014
Journal  Tissue Cell Volume  46
Issue  6 Pages  457-61
PubMed ID  25175035 Mgi Jnum  J:316195
Mgi Id  MGI:6834170 Doi  10.1016/j.tice.2014.08.002
Citation  Bogolyubova IO, et al. (2014) Immunocytochemical study of YB-1 nuclear distribution in different cell types. Tissue Cell 46(6):457-61
abstractText  In the present work we studied the distribution of YB-1 in the nuclei of mouse hepatocytes, early embryos and human skin fibroblasts with the use of light and electron microscopy. To reveal YB-1, we applied rat polyclonal antibody against the C-terminal fragment of YB-1 molecule and rabbit polyclonal antibody against full-length YB-1 molecule. YB-1 distribution patterns varied significantly in different cell types. YB-1 was found to be colocalized with RNA polymerase I in mouse hepatocytes and embryos. Besides, YB-1 was revealed in a population of Cajal bodies in 2-cell mouse embryos but not in other cells studied.
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