| First Author | Rönnberg E | Year | 2013 |
| Journal | Infect Immun | Volume | 81 |
| Issue | 6 | Pages | 2085-94 |
| PubMed ID | 23529614 | Mgi Jnum | J:199534 |
| Mgi Id | MGI:5502993 | Doi | 10.1128/IAI.00290-13 |
| Citation | Ronnberg E, et al. (2013) Granzyme D is a novel murine mast cell protease that is highly induced by multiple pathways of mast cell activation. Infect Immun 81(6):2085-94 |
| abstractText | Granzymes are serine proteases known mostly for their role in the induction of apoptosis. Granzymes A and B have been extensively studied, but relatively little is known about granzymes C to G and K to M. T cells, lymphohematopoietic stromal cells, and granulated metrial gland cells express granzyme D, but the function of granzyme D is unknown. Here we show that granzyme D is expressed by murine mast cells and that its level of expression correlates positively with the extent of mast cell maturation. Coculture of mast cells with live, Gram-positive bacteria caused a profound, Toll-like receptor 2 (TLR2)-dependent induction of granzyme D expression. Granzyme D expression was also induced by isolated bacterial cell wall components, including lipopolysaccharide (LPS) and peptidoglycan, and by stem cell factor, IgE receptor cross-linking, and calcium ionophore stimulation. Granzyme D was released into the medium in response to mast cell activation. Granzyme D induction was dependent on protein kinase C and nuclear factor of activated T cells (NFAT). Together, these findings identify granzyme D as a novel murine mast cell protease and implicate granzyme D in settings where mast cells are activated, such as bacterial infection and allergy. |
