| First Author | Ezan J | Year | 2013 |
| Journal | Nat Cell Biol | Volume | 15 |
| Issue | 9 | Pages | 1107-15 |
| PubMed ID | 23934215 | Mgi Jnum | J:201606 |
| Mgi Id | MGI:5514457 | Doi | 10.1038/ncb2819 |
| Citation | Ezan J, et al. (2013) Primary cilium migration depends on G-protein signalling control of subapical cytoskeleton. Nat Cell Biol 15(9):1107-15 |
| abstractText | In ciliated mammalian cells, the precise migration of the primary cilium at the apical surface of the cells, also referred to as translational polarity, defines planar cell polarity (PCP) in very early stages. Recent research has revealed a co-dependence between planar polarization of some cell types and cilium positioning at the surface of cells. This important role of the primary cilium in mammalian cells is in contrast with its absence from Drosophila melanogaster PCP establishment. Here, we show that deletion of GTP-binding protein alpha-i subunit 3 (Galphai3) and mammalian Partner of inscuteable (mPins) disrupts the migration of the kinocilium at the surface of cochlear hair cells and affects hair bundle orientation and shape. Inhibition of G-protein function in vitro leads to kinocilium migration defects, PCP phenotype and abnormal hair bundle morphology. We show that Galphai3/mPins are expressed in an apical and distal asymmetrical domain, which is opposite and complementary to an aPKC/Par-3/Par-6b expression domain, and non-overlapping with the core PCP protein Vangl2. Thus G-protein-dependent signalling controls the migration of the cilium cell autonomously, whereas core PCP signalling controls long-range tissue PCP. |
