| First Author | Luo W | Year | 2013 |
| Journal | J Cell Biol | Volume | 202 |
| Issue | 7 | Pages | 1057-73 |
| PubMed ID | 24081490 | Mgi Jnum | J:201808 |
| Mgi Id | MGI:5515821 | Doi | 10.1083/jcb.201210123 |
| Citation | Luo W, et al. (2013) Analysis of the local organization and dynamics of cellular actin networks. J Cell Biol 202(7):1057-73 |
| abstractText | A ctin filaments, with the aid of multiple accessory proteins, self-assemble into a variety of network patterns. We studied the organization and dynamics of the actin network in nonadhesive regions of cells bridging fibronectin-coated adhesive strips. The network was formed by actin nodes associated with and linked by myosin II and containing the formin disheveled-associated activator of morphogenesis 1 (DAAM1) and the cross-linker filamin A (FlnA). After Latrunculin A (LatA) addition, actin nodes appeared to be more prominent and demonstrated drift-diffusion motion. Superresolution microscopy revealed that, in untreated cells, DAAM1 formed patches with a similar spatial arrangement to the actin nodes. Node movement (diffusion coefficient and velocity) in LatA-treated cells was dependent on the level and activity of myosin IIA, DAAM1, and FlnA. Based on our results, we developed a computational model of the dynamic formin-filamin-actin asters that can self-organize into a contractile actomyosin network. We suggest that such networks are critical for connecting distant parts of the cell to maintain the mechanical coherence of the cytoplasm. |
