| First Author | Mahajan N | Year | 2013 |
| Journal | J Biol Chem | Volume | 288 |
| Issue | 16 | Pages | 11611-20 |
| PubMed ID | 23471964 | Mgi Jnum | J:203501 |
| Mgi Id | MGI:5527128 | Doi | 10.1074/jbc.M112.410852 |
| Citation | Mahajan N, et al. (2013) Tumor-suppressive maspin functions as a reactive oxygen species scavenger: importance of cysteine residues. J Biol Chem 288(16):11611-20 |
| abstractText | Maspin is a member of the serine protease inhibitor (serpin) superfamily and displays tumor-suppressing activity by controlling cell migration, proliferation, apoptosis, and adhesion. Here, we provide evidence that maspin acts as a reactive oxygen species (ROS) scavenger through oxidation of three structurally exposed cysteine thiols to sulfenic acid. Ablation of these cysteine residues in maspin resulted in a significant increase in total ROS production in mouse mammary TM40D cells. Also, cells containing a triple-cysteine mutant of maspin showed elevated ERK1/2 activity, a downstream target of ROS, and enhanced proliferation and colony formation. These findings establish a novel mechanism by which maspin utilizes its cysteine thiols to inhibit oxidative stress and cell growth. |
