| First Author | Pérez RJ | Year | 2013 |
| Journal | Exp Cell Res | Volume | 319 |
| Issue | 14 | Pages | 2196-204 |
| PubMed ID | 23756134 | Mgi Jnum | J:203630 |
| Mgi Id | MGI:5528409 | Doi | 10.1016/j.yexcr.2013.05.032 |
| Citation | Perez RJ, et al. (2013) Deletion of retinoic acid receptor beta (RARbeta) impairs pancreatic endocrine differentiation. Exp Cell Res 319(14):2196-204 |
| abstractText | All-trans retinoic acid (RA) signals via binding to retinoic acid receptors (RARs alpha, beta, and gamma). RA directly influences expression of Pdx1, a transcription factor essential for pancreatic development and beta-cell (beta-cell) maturation. In this study we follow the differentiation of cultured wild-type (WT) vs. RARbeta knockout (KO) embryonic stem (ES) cells into pancreatic islet cells. We found that RARbeta KO ES cells show greatly reduced expression of some important endocrine markers of differentiated islet cells, such as glucagon, islet amyloid polypeptide (Iapp), and insulin 1 (Ins1) relative to WT. We conclude that RARbeta activity is essential for proper differentiation of ES cells to pancreatic endocrine cells. |
