| First Author | Zhang X | Year | 2014 |
| Journal | J Neurosci | Volume | 34 |
| Issue | 8 | Pages | 3013-22 |
| PubMed ID | 24553941 | Mgi Jnum | J:206927 |
| Mgi Id | MGI:5553376 | Doi | 10.1523/JNEUROSCI.3681-13.2014 |
| Citation | Zhang X, et al. (2014) Peripheral role of cathepsin s in Th1 cell-dependent transition of nerve injury-induced acute pain to a chronic pain state. J Neurosci 34(8):3013-22 |
| abstractText | There is increasing evidence that CD4(+) T-cell-dependent responses are associated with the maintenance of neuropathic pain. However, little is known about the precise mechanism(s) underlying the activation of CD4(+) T-cells. We herein show that inhibition of cathepsin S (CatS) activity, either through genetic deletion or via a pharmacological inhibitor, Z-Phe-Leu-COCHO (Z-FL), significantly attenuated the maintenance of tactile allodynia, splenic hypertrophy, increased number of splenic CD4(+) T-cells and the final cleavage step of the MHC class II-associated invariant chain following peripheral nerve injury. It was also noted that splenectomy significantly attenuated the peripheral nerve injury-induced tactile allodynia, whereas the adoptive transfer of splenic CD4(+) T-cells from neuropathic wild-type mice significantly increased the pain level of splenectomized wild-type or CatS(-/-) mice. Furthermore, CatS deficiency or Z-FL treatment also significantly inhibited the infiltration of CD4(+) T-cells that expressed interferon-gamma (IFN-gamma) in the dorsal spinal cord. Signal transducer and activator of transcription 1, a molecule downstream of IFN-gamma receptor activation, was activated exclusively in microglia 7 d after peripheral nerve injury. Moreover, CatS deficiency, Z-FL treatment, or splenectomy significantly attenuated the proliferation of microglia 14 d after peripheral nerve injury. These results show a peripheral pivotal role of CatS in the development of neuropathic pain through the antigen-specific activation of CD4(+) T-cells. After activation, CD4(+) T-cells infiltrate into the dorsal spinal cord and secrete IFN-gamma to reactivate microglia, which contribute to the transition of acute pain to a chronic pain state. |
