| First Author | Tarantino N | Year | 2014 |
| Journal | J Cell Biol | Volume | 204 |
| Issue | 2 | Pages | 231-45 |
| PubMed ID | 24446482 | Mgi Jnum | J:208264 |
| Mgi Id | MGI:5562584 | Doi | 10.1083/jcb.201307172 |
| Citation | Tarantino N, et al. (2014) TNF and IL-1 exhibit distinct ubiquitin requirements for inducing NEMO-IKK supramolecular structures. J Cell Biol 204(2):231-45 |
| abstractText | Nuclear factor kappaB (NF-kappaB) essential modulator (NEMO), a regulatory component of the IkappaB kinase (IKK) complex, controls NF-kappaB activation through its interaction with ubiquitin chains. We show here that stimulation with interleukin-1 (IL-1) and TNF induces a rapid and transient recruitment of NEMO into punctate structures that are anchored at the cell periphery. These structures are enriched in activated IKK kinases and ubiquitinated NEMO molecules, which suggests that they serve as organizing centers for the activation of NF-kappaB. These NEMO-containing structures colocalize with activated TNF receptors but not with activated IL-1 receptors. We investigated the involvement of nondegradative ubiquitination in the formation of these structures, using cells deficient in K63 ubiquitin chains or linear ubiquitin chain assembly complex (LUBAC)-mediated linear ubiquitination. Our results indicate that, unlike TNF, IL-1 requires K63-linked and linear ubiquitin chains to recruit NEMO into higher-order complexes. Thus, different mechanisms are involved in the recruitment of NEMO into supramolecular complexes, which appear to be essential for NF-kappaB activation. |
