| First Author | Li H | Year | 2013 |
| Journal | Biochem Biophys Res Commun | Volume | 440 |
| Issue | 4 | Pages | 527-32 |
| PubMed ID | 24113379 | Mgi Jnum | J:211533 |
| Mgi Id | MGI:5575623 | Doi | 10.1016/j.bbrc.2013.09.095 |
| Citation | Li H, et al. (2013) Nspc1 regulates the key pluripotent Oct4-Nanog-Sox2 axis in P19 embryonal carcinoma cells via directly activating Oct4. Biochem Biophys Res Commun 440(4):527-32 |
| abstractText | Nspc1 is an identified transcription repressor. However, transiently up-regulated or down-regulated Nspc1 in P19 embryonal carcinoma cells affects expression levels of Oct4, Sox2 and Nanog in a positive correlation. Luciferase activity assays verified that Nspc1 regulates the Oct4 promoter in a dose dependent manner. ChIP assay shows that Nspc1 activates Oct4 by directly binding to the (-1021 to -784) region of Oct4 promoter. Dominant negative analysis indicated the activation is dependent on the retinoid acid response element (RARE). We demonstrated Nspc1 has a positive role in maintaining the pluripotency of P19 cells by directly regulating Oct4. |
