| First Author | García-López J | Year | 2014 |
| Journal | Biochim Biophys Acta | Volume | 1839 |
| Issue | 6 | Pages | 463-75 |
| PubMed ID | 24769224 | Mgi Jnum | J:212554 |
| Mgi Id | MGI:5581763 | Doi | 10.1016/j.bbagrm.2014.04.006 |
| Citation | Garcia-Lopez J, et al. (2014) Global characterization and target identification of piRNAs and endo-siRNAs in mouse gametes and zygotes. Biochim Biophys Acta 1839(6):463-75 |
| abstractText | A set of small RNAs known as rasRNAs (repeat-associated small RNAs) have been related to the down-regulation of Transposable Elements (TEs) to safeguard genome integrity. Two key members of the rasRNAs group are piRNAs and endo-siRNAs. We have performed a comparative analysis of piRNAs and endo-siRNAs present in mouse oocytes, spermatozoa and zygotes, identified by deep sequencing and bioinformatic analysis. The detection of piRNAs and endo-siRNAs in the spermatozoa and revealed also in zygotes, hints to their potential delivery to oocytes during fertilization. However, a comparative assessment of the three cell types indicates that both piRNAs and endo-siRNAs are mainly maternally inherited. Finally, we have assessed the role of the different rasRNA molecules in connection with amplification processes by way of the "ping-pong cycle". Our results suggest that the ping-pong cycle can act on other rasRNAs, such as tRNA- and rRNA-derived fragments, thus not only being restricted to TEs during gametogenesis. |
