| First Author | Wang Q | Year | 2014 |
| Journal | Mol Cell Biol | Volume | 34 |
| Issue | 10 | Pages | 1747-56 |
| PubMed ID | 24591653 | Mgi Jnum | J:213669 |
| Mgi Id | MGI:5585560 | Doi | 10.1128/MCB.01401-13 |
| Citation | Wang Q, et al. (2014) Homer1 alternative splicing is regulated by gonadotropin-releasing hormone and modulates gonadotropin gene expression. Mol Cell Biol 34(10):1747-56 |
| abstractText | Hypothalamic gonadotropin-releasing hormone (GnRH) plays a critical role in reproductive physiology by regulating follicle-stimulating hormone (FSH) and luteinizing hormone (LH) gene expression in the pituitary. Analysis of gonadotrope deep-sequencing data identified a global regulation of pre-mRNA splicing by GnRH. Homer1, a gene encoding a postsynaptic density scaffolding protein, was selected for further study. Homer1 expresses a short splice form, Homer1a, and more-abundant long transcripts Homer1b/c. GnRH induced a modest increase in Homer1b/c expression and a dramatic increase in the Homer1a splice form. G protein knockdown studies suggested that the Homer1 induction, but not the regulated splicing, was Galphaq/11 dependent. Phosphorylation of the splicing regulator SRp20 was found to be induced by GnRH. SRp20 depletion attenuated the GnRH-induced increase in the Homer1a-to-Homer1b/c ratio and modulated the effects of GnRH on FSHbeta and LHbeta expression. Homer1 gene knockdown resulted in increased GnRH-induced FSHbeta and LHbeta transcript levels. Furthermore, splice-form-specific reduction of Homer1b/c increased both FSHbeta and LHbeta mRNA induction, whereas reduction of Homer1a had the opposite effect on FSHbeta induction. These results indicate that the regulation of Homer1 splicing by GnRH contributes to gonadotropin gene control. |
