| First Author | Jeong BC | Year | 2014 |
| Journal | FEBS Lett | Volume | 588 |
| Issue | 18 | Pages | 3427-34 |
| PubMed ID | 25109777 | Mgi Jnum | J:214833 |
| Mgi Id | MGI:5604059 | Doi | 10.1016/j.febslet.2014.07.035 |
| Citation | Jeong BC, et al. (2014) MicroRNA-302a inhibits adipogenesis by suppressing peroxisome proliferator-activated receptor gamma expression. FEBS Lett 588(18):3427-34 |
| abstractText | The present study explored the involvement of miR-302a in adipocyte differentiation via interaction with 3'-untranslated region of peroxisome proliferator-activated receptor gamma (PPARgamma) mRNA. In differentiating 3T3-L1 adipocytes, expression of miR-302a was negatively correlated with that of the adipogenic gene aP2 and PPARgamma. Overexpression of miR-302a inhibited adipogenic differentiation with lipid accumulation, and inversely anti-miR-302a increased the differentiation. In silico analysis revealed a complementary region of miR-302a seed sequence in 3'-UTR of PPARgamma mRNA. Luciferase assay showed the direct interaction of miR-302a with PPARgamma at the cellular level. The miR-302a inhibition of adipocyte differentiation was reversed by PPARgamma overexpression. These findings suggest that miR-302a might be a negative regulator of adipocyte differentiation and that the dysregulation of miR-302a should lead to metabolic disorders. |
