| First Author | Taylor J | Year | 2014 |
| Journal | J Cell Biol | Volume | 205 |
| Issue | 6 | Pages | 829-46 |
| PubMed ID | 24934157 | Mgi Jnum | J:215792 |
| Mgi Id | MGI:5606252 | Doi | 10.1083/jcb.201403021 |
| Citation | Taylor J, et al. (2014) The Cavbeta1a subunit regulates gene expression and suppresses myogenin in muscle progenitor cells. J Cell Biol 205(6):829-46 |
| abstractText | Voltage-gated calcium channel (Cav) beta subunits are auxiliary subunits to Cavs. Recent reports show Cavbeta subunits may enter the nucleus and suggest a role in transcriptional regulation, but the physiological relevance of this localization remains unclear. We sought to define the nuclear function of Cavbeta in muscle progenitor cells (MPCs). We found that Cavbeta1a is expressed in proliferating MPCs, before expression of the calcium conducting subunit Cav1.1, and enters the nucleus. Loss of Cavbeta1a expression impaired MPC expansion in vitro and in vivo and caused widespread changes in global gene expression, including up-regulation of myogenin. Additionally, we found that Cavbeta1a localizes to the promoter region of a number of genes, preferentially at noncanonical (NC) E-box sites. Cavbeta1a binds to a region of the Myog promoter containing an NC E-box, suggesting a mechanism for inhibition of myogenin gene expression. This work indicates that Cavbeta1a acts as a Cav-independent regulator of gene expression in MPCs, and is required for their normal expansion during myogenic development. |
