| First Author | Soni S | Year | 2014 |
| Journal | Proc Natl Acad Sci U S A | Volume | 111 |
| Issue | 37 | Pages | 13337-42 |
| PubMed ID | 25197097 | Mgi Jnum | J:216491 |
| Mgi Id | MGI:5608867 | Doi | 10.1073/pnas.1405422111 |
| Citation | Soni S, et al. (2014) Transcription factor EKLF (KLF1) recruitment of the histone chaperone HIRA is essential for beta-globin gene expression. Proc Natl Acad Sci U S A 111(37):13337-42 |
| abstractText | The binding of chromatin-associated proteins and incorporation of histone variants correlates with alterations in gene expression. These changes have been particularly well analyzed at the mammalian beta-globin locus, where transcription factors such as erythroid Kruppel-like factor (EKLF), which is also known as Kruppel-like factor 1 (KLF1), play a coordinating role in establishing the proper chromatin structure and inducing high-level expression of adult beta-globin. We had previously shown that EKLF preferentially interacts with histone H3 and that the H3.3 variant is differentially recruited to the beta-globin promoter. We now find that a novel interaction between EKLF and the histone cell cycle regulation defective homolog A (HIRA) histone chaperone accounts for these effects. HIRA is not only critical for beta-globin expression but is also required for activation of the erythropoietic regulators EKLF and GATA binding protein 1 (GATA1). Our results provide a mechanism by which transcription factor-directed recruitment of a generally expressed histone chaperone can lead to tissue-restricted changes in chromatin components, structure, and transcription at specific genomic sites during differentiation. |
