| First Author | Zhu F | Year | 2014 |
| Journal | Exp Cell Res | Volume | 327 |
| Issue | 2 | Pages | 256-63 |
| PubMed ID | 24798100 | Mgi Jnum | J:217576 |
| Mgi Id | MGI:5614552 | Doi | 10.1016/j.yexcr.2014.04.018 |
| Citation | Zhu F, et al. (2014) The nuclear factor kappa B (NF-kappaB) activation is required for phagocytosis of staphylococcus aureus by RAW 264.7 cells. Exp Cell Res 327(2):256-63 |
| abstractText | Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor which controls the expression of various genes involved in immune responses. However, it is not clear whether NF-kappaB activation is critical for phagocytosis when Staphylococcus aureus is the pathogen. Using oligonucleotide microarrays, we investigated whether NF-kappaB cascade genes are altered in a mouse leukemic monocyte macrophage cell line (RAW 264.7) when the cells were stimulated to activate a host innate immune response against live S. aureus or heat-inactivated S. aureus (HISA). NF-kappaB cascade genes such as Nfkappab1, Nfkappabiz, Nfkappabie, Rel, Traf1 and Tnfaip3 were up-regulated by all treatments at one hour after incubation. NF-kappaB play an important role in activating phagocytosis in RAW 264.7 cells infected with S. aureus. Inhibition of NF-kappaB significantly blocked phagocytosis of fluorescently labeled S. aureus and decreased the expression of NFkappaB1, IL1alpha, IL1beta and TLR2 in this cell line. Our results demonstrate that S. aureus may activate the NF-kappaB pathway and that NF-kappaB activation is required for phagocytosis of S. aureus by macrophages. |
