| First Author | Wang X | Year | 2015 |
| Journal | FEBS Lett | Volume | 589 |
| Issue | 4 | Pages | 490-6 |
| PubMed ID | 25613181 | Mgi Jnum | J:217653 |
| Mgi Id | MGI:5615289 | Doi | 10.1016/j.febslet.2015.01.007 |
| Citation | Wang X, et al. (2015) MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells. FEBS Lett 589(4):490-6 |
| abstractText | Low-density lipoprotein receptor (LDLR) catalyzes the uptake of LDL-cholesterol by liver and peripheral organs. The function of the LDLR is antagonized by pro-protein convertase subtilisin/kexin type 9 (PCSK9), which binds to LDLR at the plasma membrane inducing LDLR degradation. Here, we report that matrix metalloproteinase-2 (MMP-2) interacts with and cleaves PCSK9, as evidenced by proteomic, chemical cross-linkage, blue native-PAGE and domain-specific antibodies Western blot analyses. Furthermore, MMP-2 overexpression renders Hepa1-c1c7 cells resistant to PCSK9-induced LDLR degradation. The data suggest that pathological MMP-2 overexpression may protect the LDLR from PCSK-9-induced degradation. |
