| First Author | Schücker K | Year | 2015 |
| Journal | Proc Natl Acad Sci U S A | Volume | 112 |
| Issue | 7 | Pages | 2029-33 |
| PubMed ID | 25646409 | Mgi Jnum | J:220014 |
| Mgi Id | MGI:5632033 | Doi | 10.1073/pnas.1414814112 |
| Citation | Schucker K, et al. (2015) Elucidation of synaptonemal complex organization by super-resolution imaging with isotropic resolution. Proc Natl Acad Sci U S A 112(7):2029-33 |
| abstractText | Synaptonemal complexes (SCs) are meiosis-specific multiprotein complexes that are essential for synapsis, recombination, and segregation of homologous chromosomes, but the molecular organization of SCs remains unclear. We used immunofluorescence labeling in combination with super-resolution imaging and average position determination to investigate the molecular architecture of SCs. Combination of 2D super-resolution images recorded from different areas of the helical ladder-like structure allowed us to reconstruct the 3D molecular organization of the mammalian SC with isotropic resolution. The central element is composed of two parallel cables at a distance of approximately 100 nm, which are oriented perpendicular to two parallel cables of the lateral element arranged at a distance of approximately 220 nm. The two parallel cable elements form twisted helical structures that are connected by transversal filaments by their N and C termini. A single-cell preparation generates sufficient localizations to compile a 3D model of the SC with nanometer precision. |
