| First Author | Lee HS | Year | 2014 |
| Journal | Biochem Biophys Res Commun | Volume | 452 |
| Issue | 4 | Pages | 1084-90 |
| PubMed ID | 25245289 | Mgi Jnum | J:220081 |
| Mgi Id | MGI:5632218 | Doi | 10.1016/j.bbrc.2014.09.055 |
| Citation | Lee HS, et al. (2014) Shedding of epithin/PRSS14 is induced by TGF-beta and mediated by tumor necrosis factor-alpha converting enzyme. Biochem Biophys Res Commun 452(4):1084-90 |
| abstractText | Epithin/PRSS14, a type II transmembrane serine protease, plays critical roles in cancer metastasis. Previously, we have reported that epithin/PRSS14 undergoes ectodomain shedding in response to phorbol myristate acetate (PMA) stimulation. In this study, we show that transforming growth factor-beta (TGF-beta) induces rapid epithin/PRSS14 shedding through receptor mediated pathway in 427.1.86 thymoma cells. Tumor necrosis factor-alpha converting enzyme (TACE) is responsible for this shedding. Amino acid sequence encompassing the putative shedding cleavage site of epithin/PRSS14 exhibit strong homology to the cleavage site of l-selectin, a known TACE substrate. TACE inhibitor, TAPI-0 and TACE siRNA greatly reduced TGF-beta-induced epithin/PRSS14 shedding. TGF-beta treatment induces translocation of intracellular pool of TACE to the membrane where epithin/PRSS14 resides. These findings suggest that TGF-beta induces epithin/PRSS14 shedding by mediating translocation of epithin/PRSS14 sheddase, TACE, to the membrane. |
