| First Author | Ji S | Year | 2014 |
| Journal | Biochem Biophys Res Commun | Volume | 451 |
| Issue | 2 | Pages | 329-33 |
| PubMed ID | 25094047 | Mgi Jnum | J:220114 |
| Mgi Id | MGI:5632251 | Doi | 10.1016/j.bbrc.2014.07.130 |
| Citation | Ji S, et al. (2014) PU.1 promotes miR-191 to inhibit adipogenesis in 3T3-L1 preadipocytes. Biochem Biophys Res Commun 451(2):329-33 |
| abstractText | PU.1, an Ets family transcription factor, was previously demonstrated expressed in 3T3-L1 preadipocytes and had an negative effect on adiopogenesis. However, the underlying mechanism remains elusive. Here, miR-191 was identified as an inhibitor of adipocyte differentiation through targeting the 3' untranslated regions of C/EBPbeta, the initial factor in the C/EBPalpha/beta-PPARgamma terminal pathway of adipogenic differentiation. MiR-191 suppressed the lipid accumulation by Oil Red O staining and downregulated the levels of adipogenic marker genes PPARgamma (P<0.01), aP2 (P<0.01) and FAS (P<0.05). Then, we found that PU.1 overexpression resulted in upregulation of miR-191 and adipogenic inhibition. Likewise, PU.1 overexpression rescued the miR-191 decrease and resisted the adipogenic promotion caused by miR-191 oligonucleotide inhibitor. Collectively, these results revealed that PU.1 promoted miR-191 to suppress adipogenesis 3T3-L1 preadipocyte and indicated a new mechanism of PU.1 inhibiting adipogenesis. |
