| First Author | Hadigal SR | Year | 2015 |
| Journal | Nat Commun | Volume | 6 |
| Pages | 6985 | PubMed ID | 25912399 |
| Mgi Jnum | J:222795 | Mgi Id | MGI:5645610 |
| Doi | 10.1038/ncomms7985 | Citation | Hadigal SR, et al. (2015) Heparanase is a host enzyme required for herpes simplex virus-1 release from cells. Nat Commun 6:6985 |
| abstractText | Herpesviruses exemplified by herpes simplex virus-1 (HSV-1) attach to cell surface heparan sulfate (HS) for entry into host cells. However, during a productive infection, the HS moieties on parent cells can trap newly exiting viral progenies and inhibit their release. Here we demonstrate that a HS-degrading enzyme of the host, heparanase (HPSE), is upregulated through NF-kB and translocated to the cell surface upon HSV-1 infection for the removal of HS to facilitate viral release. We also find a significant increase in HPSE release in vivo during infection of murine corneas and that knockdown of HPSE in vivo inhibits virus shedding. Overall, we propose that HPSE acts as a molecular switch for turning a virus-permissive 'attachment mode' of host cells to a virus-deterring 'detachment mode'. Since many human viruses use HS as an attachment receptor, the HPSE-HS interplay may delineate a common mechanism for virus release. |
