| First Author | Mi W | Year | 2016 |
| Journal | FEBS Lett | Volume | 590 |
| Issue | 3 | Pages | 396-407 |
| PubMed ID | 26785690 | Mgi Jnum | J:229974 |
| Mgi Id | MGI:5755181 | Doi | 10.1002/1873-3468.12064 |
| Citation | Mi W, et al. (2016) miR-33a-5p modulates TNF-alpha-inhibited osteogenic differentiation by targeting SATB2 expression in hBMSCs. FEBS Lett 590(3):396-407 |
| abstractText | miRNAs play a number of roles in bone, including mediating the pathological effects of inflammation. Here, we found that miR-33a-5p expression was significantly increased after TNF-alpha treatment during BMP-2-induced osteogenic differentiation of hBMSCs. Luciferase reporter assays and western blotting demonstrated that special AT-rich sequence-binding protein 2 (SATB2) is a target of miR-33a-5p. Moreover, we show that BMP-2 induces SATB2 expression by interacting with SATB2 directly via the BMP-2-RUNX2 pathway. However, TNF-alpha first decreases SATB2 expression by inhibiting miR-33a-5p degradation. We thus conclude that miR-33a-5p plays a central role in this complex regulatory network. These findings will help to understand the regulatory role of miR-33a-5p in the inflammatory process. |
